Strain discrimination of Yersinia pestis using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences† †Electronic supplementary information (ESI) available: Electrochemical melting curves along with 95% confidence intervals for the three PCR amplicons hybridized to probe-1, fluorescence melting curves for amplicons hybridized to probe-2 in the presence of a blocker oligonucleotide, structure of the thiol linker. See DOI: 10.1039/c4sc03084b
نویسندگان
چکیده
Chemistry, University of Southampton, H E-mail: [email protected] ATDBio Ltd, Chemistry, University of Sout 1BJ, UK DSTL, Wiltshire SP4 0JQ, Salisbury, Porton Department of Chemistry, University of O Oxford OX1 3TA, UK † Electronic supplementary information ( curves along with 95% condence int hybridized to probe-1, uorescence melti probe-2 in the presence of a blocker o linker. See DOI: 10.1039/c4sc03084b Cite this: Chem. Sci., 2015, 6, 1846
منابع مشابه
Strain discrimination of Yersinia pestis using a SERS-based electrochemically driven melting curve analysis of variable number tandem repeat sequences.
Strain discrimination within genetically highly similar bacteria is critical for epidemiological studies and forensic applications. An electrochemically driven melting curve analysis monitored by SERS has been utilised to reliably discriminate strains of the bacterial pathogen Yersinia pestis, the causative agent of plague. DNA amplicons containing Variable Number Tandem Repeats (VNTRs) were ge...
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More than 20 years after the first in vitro amplification of DNA by PCR, this technique is firmly established for diagnostic purposes in many clinical laboratories. PCR technology is an exciting field that has continually advanced. Evolutionary steps have been, for example, the transition from conventional cycling to rapid-cycling (1 ), from post-PCR to real-time product analysis (2 ), and from...
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Pages List of Tables and Figures ........................................................................................................ S1 General ..................................................................................................................................... S2 Syntheses of 5’ boronooligonucleotides ...........................................................................
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عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2015